Collagen fibers in cartilage, E. I. Romijn, NTNU, Trondheim Eudorina - Pavel Skaloud, Charles University, Věda je krásná1- CVD grown diamond film, Magdalena Parlinska, University of Rzeszow, Poland
Scabiosa columbaria - Viktor Sykora, Charles University, Věda je krásnáLeaf Fract, TESCAN1- Diatom, Magdalena Parlinska, University of Rzeszow, Poland
Rust fungus spore,Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina.jpg 2- Diatom, Magdalena Parlinska, University of Rzeszow, Poland Salt, TESCAN
Plasma coating crossection, TESCANOrchid root showing with idioblastic cells, S. R. Senthilkumar, St. Joseph´s College, India Rhaphoneis - Pavel Skaloud, Charles University, Věda je krásná
Energy filtered TEM micrograph of yttria (in green) - zirconia (in red) multilayers, Chanchal Ghosh,  IGCAR, Kalpakkam, India  Butterfly Wings, Benedykt R. Jany, Marian SmoluchowskiInstitute of Physics - Jagiellonian University, Poland Offretite Scagno, TESCAN
Cross section of an Abutilon leaf, Adriana Dominguez and Eduardo Favret, CNEA - INTA, Argentina Paulinella chromatophora - Yvonne Nemcova, Charles University, Věda je krásnáCoral, TESCAN
4- Gel beads coated with a RuC13 coatings, Magdalena Parlinska, University of Rzeszow, Poland Chroococcus giganteus - Jan Stastny, Charles University, Věda je krásnáScenedesmus quadricauda - Viktor Sykora, Charles University , Věda je krásná

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Thursday, 11 September
Time: 12:30 - 13:45

GE Healthcare (45 min)
Room: Meeting Hall 4 (2nd floor)

12.30 - 12.45 Welcome Lunch Provided

12.45 - 13.30 Practical Approaches and Considerations for Super-resolution Imaging. Daniel J White Ph.D. & Przemyslaw Fleszar, Field Applications Scientist GE Healthcare Life Sciences.

3D-SIM and Localization microscopy are powerful techniques for cell biologists. When viewed with a microscope, the signal from any sub-diffraction-limit object (e.g., a single fluorescent molecule) will be blurred because of diffraction, thus causing the object to appear larger than its actual size. Recently a variety of techniques have been developed to allow researchers to be able to get a closer look at the actual objects size and location. Collectively these techniques are referred to as Optical Super-resolution.

3D-SIM is a technique that utilizes a structured light pattern and image reconstruction to gain a two-fold resolution improvement in XY and Z. Localization microscopy represents a combination of imaging and mathematical modelling, which can be used to precisely determine the position of fluorophores in a biological sample. GE Healthcare's DeltaVision Microscopy Systems offer options for both these imaging modalities. However instrumentation is only part of the solution. Electron microscopists have known for decades that the more resolution you seek the more the characteristics of the sample and preparation methods become critical. Our presentation will give a brief overview of 3D-SIM and Localization Microscopy, discussing the practical considerations necessary to obtain the best results from optical super-resolution techniques and when to use them.

13.30 - 13.45 Questions

 

 





 

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